Microscale controlled continuous cell culture
نویسنده
چکیده
Continuous culture experiments provide steady state conditions where cellular activity can be measured reproducibly. Experiments are generally prohibitive due to the cost and volume of growth media for long term experiments. Microfluidic devices are well suited to reduce volume requirements for experiments, but controlling volume in elastic devices is challenging. We propose a plastic-PDMS device for integrating valves on chip while maintaining structural rigidity. The device demonstrates flow control, mixing, and volume consistency over long term operation and an example 2 week long continuous culture experiment is shown.
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